EFEK SITOTOKSIK BEBERAPA AKAR BAJAKAH KALIMANTAN TERHADAP SEL KANKER PAYUDARA T47D
DOI:
https://doi.org/10.21831/jps.v26i2.41211Keywords:
bajakah, Uncaria, Spatholobus, Schefflera, T47DAbstract
CYTOTOXIC EFFECT OF KALIMANTAN BAJAKAH ROOTS ON T47D BREAST CANCER CELLS
There are 200 kinds of bajakah in Indonesia; which bajakah tampala, kalalawit, bajakah jari lima, and longkur which have been used as herbal medicine of anti-breast cancer by Dayak ethnic. This study was to find out the cytotoxicity of four bajakah extracts toward T47D cell lines with tamoxifen as a positive control. Bajakah extracts of aqua, hot aqua, ethanol 96%, and n-hexane were carried out using a sample-grinding pestle. The IC50 resulted were 1136; 1871.5; 2294.25; and 3975.5 µg/mL (R2 = 0.962; 0.943; 0.943; and 0.956) respectively for aqua, hot aqua, ethanol 96%, and n-hexane extracts; 9 µg/mL (R2 = 0.9581) for tamoxifen. The ethanolic extract was the most toxic compared to other extracts. Therefore, to improve the IC50, reflux extraction was conducted using ethanol 96% to four bajakahs. The improved IC50 were 407; 708; 881; and 1096 µg/mL (R2 = 0.9717; 0.952; 0.9367; 0.9369) for respectively bajakah kalalawit, tampala, longkur, and jari lima. It is concluded that the extracts contained active breast-anticancer substances because all the R2 results were >0.93.
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