ACTIVITY OF SITOTOKSIK ETANOL EXTRACT PLANT GROWTH (Cynometra ramiflora Linn) ON HEAVY CELL, T47D and WiDR
DOI:
https://doi.org/10.21831/jps.v18i2.2137Keywords:
Cynometra ramiflora, cytotoxicity, HeLa, IC50, T47D, WiDrAbstract
Cynometra ramifolia Linn is known as Sala plant. This plant, especially in Solo City categorized as rare plant and traditionaly used to cure uric acid, diabetes, hypertension, and others pain. It is interesting to know the other activities by done the test for human cancer cell-lines. This research objective to identify the cytotoxicity effects of ethanol extract from C.ramiflora the stem bark and leaves against T47D, HeLa and WiDr cancer cell lines. The cytotoxicity test of ethanol extract from C.ramiflora the stem bark and leaves determined by MTT assay by calculating the level of IC50 which was based on the percentage of the cell death following the 24 hours incubation with the extract. The results showed that ethanol extract of stem bark has cytotoxic effect to HeLa, T47D and WiDr cell-lines with the IC50 of >1000; 0,90 and 6,29 g/mL respectively. The leave cytotoxic effect to HeLa, T47D and WiDr cell-lines with the IC50 of 1,92; 6,37 and 0,41 g/mL respectively. This research indicated that the ethanol extract isolated from C. Ramiflora leaves a selective cytotoxicity effect to WiDr cell line.
References
Bunyapraphatsara, Nuntavan, Jutiviboon-suk, A., Sornlek, P., Therathanathorn, W., Aksornkaew, S., Fong, Harry H.S., Pezzuto, John M., and Kosmeder, Jerry. 2003. Pharmacological studies of plants in the mangrove forest. India: Mahidol University.
Cho, S.J, Valerie, H.L., Wu-Sing, S.H., Sing, K.Y., Pereira, J.P., dan Goh, S.H. xanthones from garcinia gaundichaudii 1998. Novel cytotoxic polyprenilaterd xanthones from garcinia gaundichaudii (Guttiferae), Tetrahedron, 54: 1091510924.
Dave, R. 2006. Mangrove ecosystem of south, west Madagascar: an ecological, human impact, and subsistence value assessment. Tropical Resources Bulletin 25: 7-13.
Ditjen INTAG. 1993. Hasil penafsiran luas areal dari citra landsat MSS liputan tahun 1986-1991. Direktorat Jenderal Inventarisasi dan Tata Guna Hutan, Departemen Kehutanan RI.
Ditjen RLPS. 2001. Kriteria dan standar teknis rehabilitasi wilayah pantai. Direktorat Jenderal Rehabilitasi Lahan dan Perhutanan Sosial, Departemen Kehutanan RI.
Djajanegara, I., dan Wahyudi, P. 2009. Pemakaian sel HeLa dalam uji sitotoksisitas fraksi kloroform dan etanol ekstrak daun annona squamosa, Jurnal Ilmu Kefarmasian Indonesia, Vol.7., No.1, 7-11.
Doyle, A. & Griffith, S.J.B. 2000. Cell and tissue culture for medical research, 49. New York: John Willey and Sons, Ltd.
Fessenden, R.J., and Fessenden, J.S. 1994. Organic Chemistry, 5th Edition, Willard Grant Press, Boston.
Haryoto. 2013. Teknik uji hayati untuk pengembangan obat (TUHPO). Surakarta: Fairuz Media.
Khan, Ali, M.A., Prasanta Paul, P., and Islam, M.T. 2006. Phytochemical and pharmacological screening of Shingra (Cynometra ramiflora Linn., Family: Leguminosae) bark based on its traditional uses. Department of Pharmacy Southern University.
Loomis, T., 1978. Essential of toxicology, 3rd edition. Philadelpia: Lea & Febriger.
Onrizal. 2005. Hutan mangrove selamatkan masyarakat di pesisir utara Nias dari tsunami. Warta Konservasi Lahan Basah 13 (2): 5-7.
Suhendi. A., Haryoto, Indrayudha, P., Muhtadi, Azizah, T. 2013. Determination of antioxidant activity of extract polar and semipolar fraction of cynometra ramiflora Linn using DPPH assay. Acta Pharmacie Indonesia, Vol.1, No.1, Maret 2013.
Tiwari. P., Rahuja. N., Kumar, R., Lakshmi, V., Srivastava, N.M., Agarwal, C.S., Raghubir, R., and Srivastava, K.A. 2008. Search for antihyperglycemic activity in few marine flora and fauna. Indian Journal of Science andTechnology. 1 (5), p.1-5.
Uddin, Shaikh, J., Grice Darren, I., and Tiralongo, E. 2009. Cytotoxic effects of bangladeshi medicinal plant extracts. original article. eCAM Advance Access published August 25, P.1-6.
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