ISOLATION OF ENDOPHYTIC BACTERIA FROM SOURSOP LEAF (Annona muricata) AND SECONDARY ANTIOXIDANT METABOLIT ACTIVITY TESTING

Purbowatiningrum Ria Sarjono

doi:10.21831/jps.v1i1.57799

ISOLATION OF ENDOPHYTIC BACTERIA FROM SOURSOP LEAF (Annona muricata) AND SECONDARY ANTIOXIDANT METABOLIT ACTIVITY TESTING

Purbowatiningrum Ria Sarjono, Diponegoro University, Indonesia

10.21831/jps.v1i1.57799

Abstract

Free radicals are molecules or molecular fragments that contain unpaired electrons in the valence shell. To achieve stability, free radical molecules will react with surrounding molecules. Free radical molecules in the body take place continuously and are difficult to stop, this can cause diseases such as premature aging and cancer. Therefore, the required compound has antioxidant activity to stop it. One of the antioxidant compounds can be obtained by utilizing secondary metabolites of Soursop leaf endophytic bacteria (Annona muricata). Soursop leaf endophytic bacteria isolates have been obtained which have the form of diplobacillus and include gram-positive bacteria. The activity was carried out using the DPPH method and obtained IC₅₀ of 278.27 ppm at the 22nd hour and 61.942 ppm at the 24th hour. The total phenolic obtained at the 22nd hour was 79 mgek of gallic acid/g while at the 24th hour it was 34 mgek of gallic acid/g. Secondary metabolites of soursop leaf endophytic bacteria produce secondary metabolites in the form of alkaloids and terpenoids.

Keywords

Annona muricata, endophytic bacteria, secondary metabolites, antioxidants, DPPH.

Full Text:

PDF

References

Asbanu, Yoseanno Widi Anugrah, Nanik Wijayati, dan Ersanghono Kusumo. 2019. “Identifikasi Senyawa Kimia Ekstrak Daun Sirsak (Annona muricata L.) dan Uji Aktivitas Antioksidannya dengan Metode DPPH (2,2-Difenil-1-Pikrilhidrasil).” Indonesian Journal of Chemical Science 8(3).

Dhurhania, Crescentiana Emy, dan Agil Novianto. 2018. “Uji Kandungan Fenolik Total dan Pengaruhnya terhadap Aktivitas Antioksidan dari Berbagai Bentuk Sediaan Sarang Semut (Myrmecodia pendens).” Jurnal Faramasi dan Ilmu Kefarmasian Indonesia 5(2).

Hasmila, H. Natsir, dan N. H. Soekamto. 2019. “Phytochemical analysis and Antioxidant Activty of Sorsop Leaf extract (Annona muricata Linn.).” Journal of Physics: Conference Series.

Indra, Novianti Nurmalasari, dan Meti Kusmiati. 2019. “Fenolik Total, Kandungan Flavonoid, dan Aktivitas Antioksidan Ekstrak Etanol Daun Mareme (Glochidion arborescens Blume.” J u r n a l S a i n s F a r m a s i & K l i n i s 6(3):206–12.

Ionita, Petre. 2021. “The Chemistry of DPPH Free Radical and Congeners.” MDPI 22(4). doi: https://doi.org/10.3390/ijms22041545.

Michalak, Monika. 2022. “Plant-Derived Antioxidants: Significance in Sin and the Ageing Process.” MDPI 23(2).

Munteanu, Irina Georgiana, dan Constantin Apetrei. 2021. “Analytical Methods Used in Determining Antioxidant Activity:A Review.” Int J Mol Sci 22(7).

Noguchi, Noriko, dan Etsuo Niki. 2019. “Chemistry of Active Oxygen Species and Antioxidant.” in Antioxidant Status, Diet, Nutrition, and Health, diedit oleh A. M. Papas. United State: CRC Press.

Pratiwi, Brasti Eka. 2015. “Isolasi dan Skrining Fitokimia Bakteri Endofit Dari Daun Rambutan (Nephelium lappaceum L.) yang Berpotensi Sebagai Antibakteri.”

Pratiwi, Denia, dan Isna Wardaniati. 2019. “Pengaruh Variasi Perlakuan (Segar dan Simplisia) Rimpang Kunyit (Curcuma domestica) Terhadap Aktivitas Antioksidan dan Kadar Fenol Total.” Jurnal Farmasi Higea 11(2).

Prayoga, Dewa Gede Eka, Komang Ayu Nocianitri, dan Ni Nyoman Puspawati. 2019. “Identification of Phytochemical Compounds and Antioxidant Activity of Pepe Leaves (Gymnema reticulatum Br.) Crude Extract in Various Solvent Types.” Jurnal Ilmu dan Teknologi Pangan 8(2):111–21.

Putri, Dayu Nirwana, Sri Widyarti, dan Yoga Dwi Jatmiko. 2022. “Increasing Antioxidant Activity of Soursoup (Annona muricata L.) and Noni (Morinda citrifolia L.) Leaves Fermented by Lactobacillus plantarum P1022.” Berkala Penelitian Hayati 27(2).

Rahmawati, S. I., F. N. Izzati1, Y. Hapsari1, E. Septiana1, F. Rachman1, Bustanussalam, dan P. Simanjuntak1. 2019. “Endophytic Microbes and Antioxidant Activities of Secondary Metabolites from Mangroves Avicennia marina and Xylocrapus granatum.” IOP Conference Series: Earth and environmental Science.

Rasyadi, Yahdian, Farida Rahim, dan Silvia Devita. 2022. “Aktivitas Antioksidan Handbody Lotion Ekstrak Etanol Daun Sirsak (Annona muricata Linn) dengan Metode DPPH.” Jurnal Ilmiah Farmasi 11(2).

Simanjuntak, Krisitina. 2012. “Peran Antioksidan Dalam Meningkatkan Kesehatan.” Bina Widya 23(3).

Siyanti, Amelia, Nurul Fitriani, dan Angga Cipta Narsa. 2019. “Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Alpukat (Persea americana Mill.) terhadap Peredaman.” Mulawarman Pharmaceuticals Conferences.

Sunday, Rita M., dan Julius O. Oyedele. 2017. “Effect of Anthocleista vogelii leaves on Free Radicals:Antipxidant Studies.” Intenational Journal of Advanced Bochemistry Research 1(1):38–42.

Wahyuningsih, N., dan E. Zulaika. 2018. “Perbandingan Pertumbuhan akteri Selulolitik Pada Media Nutrient Broth dan Carboxy Methyl Cellulose.” JURNAL SAINS DAN SENI ITS 7(2).

White, James F., Kathryn L. Kingsley, Qiuwei Zhang, Rajan Verma, Nkolika Obi, Sofia Dvinskikh, Matthew T. Elmore, Satish K. Verma, Surendra K. Gondband, dan Kurt P. Kowalski. 2019. “Review: Endophytic Microbes and Their Potential Application In Crop Management.” SCI.

Widyasanti, Asri, Dadan Rohdiana, dan Novriana Ekatama. 2016. “Aktivitas Antioksidan Ekstrak Teh Putih (Camellia sinensis) Dengan Metode DPPH (2,2 Difenil-1-Pikhrilhidrazil).” 1(1).

Wulandari, Destik, dan Desi Purwaningsih. 2019. “Identifikasi dan Karakterisasi Bakteri Amilolitik Pada Umbi Colocasia esculenta L. Secara Morfologi, Biokimia, dan Molekuler.” Bioteknologi dan Biosains Indonesia 6(2).

DOI: https://doi.org/10.21831/jps.v1i1.57799