Email this article BIODEGRADASI MASKER MEDIS DAN NONMEDIS MENGGUNAKAN ISOLAT BAKTERI DALAM KOLOM WINOGRADSKY
Abstract
Sampah masker merupakan sampah infeksius dan sulit terurai di alam sehingga dampaknya berbahaya bagi lingkungan. Salah satu strategi untuk mengendalikan dampak sampah masker adalah biodegradasi. Penelitian ini bertujuan mengetahui perbedaan kemampuan degradasi masker medis dan nonmedis oleh 2 isolat bakteri terseleksi sebagai pendegradasi masker dalam kolom winogradsky yang diinkubasi pada kondisi lingkungan berbeda. Kolom winogradsky diinkubasi selama 40 hari dengan parameter yang diukur adalah jumlah dan kerapatan bakteri, pH, dan kehilangan massa masker setelah inkubasi. Data dianalisis dengan ANOVA α=0,05 menunjukkan bahwa jenis inokulum dan kondisi lingkungan berpengaruh signifikan terhadap nilai degradasi masker medis dan nonmedis. Uji DMRT menunjukkan bahwa inokulum terbaik dalam mendegradasi masker yaitu pada kondisi terang yaitu konsorsium AB dengan rerata persentase degradasi masker medis dan nonmedis secara berurutan 1,9% dan 3,24%, diikuti inokulum A sebesar 1,84% dan 2,69%; inokulum B sebesar 0,97% dan 1,29%. Setelah uji biodegradasi selama 40 hari terlihat bahwa inokulum A, B, AB, dan kontrol lumpur steril secara berurutan memiliki rerata jumlah koloni 2,95 x 1012 CFU/ml, 7,35 x 1011 CFU/ml, 5,96 x 1012 CFU/ml, dan 0 CFU/ml. Sementara itu, rerata nilai opticl density secara berurutan juga yaitu 0,240; 0,235; 0,249; dan 0,105.
Kata kunci: Biodegradasi, masker, kolom winogradsky
Keywords
References
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DOI: https://doi.org/10.21831/jsd.v13i1.65994
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