Frida Rosenova, Universitas Muhammadiyah Surakarta, Indonesia
Haryoto Haryoto, Unviersitas Muhammadiyah Surakarta, Indonesia
Andi Suhendi, Universitas Muhammadiyah Surakarta, Indonesia


Antibacterial activity in vivo study of ethanol extract nonpolar fraction of inggu stem (Ruta angustifolia [L.] Pers) was conducted on Staphylococcus aureus and Streptococcus mutans. It had a purpose to determine the effect of non polar fraction of ethanol extract of inggu stem on animals that had been infected by bacteria Staphylococcus aureus and Streptococcus mutans. Inggu stem powder was macerated with ethanol 96% and fractionated by vacuum liquid chromatography using hexane : chloroform 6:4, 5:5, 4:6, 3:7. Inggu stem was tested with in vivo antibacterial activity on mice with various doses of 0.3, 1.2 and 2.14 g/kg. Intraperitoneal fluid of mice had been treated was cultured on agar media and colonies of formed bacteria were counted. Antibacterial activity on Staphylococcus aureus bacteria has an average percentage of inhibition for colony are respectively 73, 81 and 97 %., whereas that of the Streptococcus mutans bacteria are 41,81 and 97% respectively. Identification of thin layer chromatography fraction in nonpolar compounds of ethanol extract of inggu stem is indicated by flavonoids, terpenoids, and alkaloids.




Antibacterial; Ruta angustifolia L., S. aureus, S. Mutans and alkaloids.

Full Text:



Agoes, A. 2010 Tanaman obat Indonesia, Buku 3, 25-26. Jakarta: Penerbit Salemba Medika.

Artika, I.M., Susilo, H., Setyo, A.V.D.,Hasan, A.E.Z. 2011. Antibacterial activity of propolis supplemented-chewing candy againts streptococcus mutans.Microbiology Indonesia, Vol 5, No 3.

Asgarpanah, J. and Khoshkam, R. 2012. Phytochemistry and pharmacological properties of Ruta graveolens L., Journal of Medicinal Plants Research,Vol 6(23), 3942-3949.

Baumert, A., Groger, D., Kuzofkina, I.N., Reisch, J. 1992. Secondary metabolites produced by callus cultures of various ruta species. Plant Cell, Tissue and Organ Culture, Vol 28, 159-162.

Benazir, J.F, Suganthi, R., Renjini, D.M.R., Suganya,K., Monisha, K., Nilzar, A., et al. 2011. Phytochemical profiling, Antimicrobial and Cytotoxicity Studies of Methanolic Extracts from Ruta graveolens, Journal of Pharmacy Research, 4(5), 1407-1409.

Gunaydin, K. and Savchi, S. 2005. Phytochemical Studies on Ruta Chalapensis (Lam.) Lamarck, Natural Product Research, Vol 19, No 3, 203-210.

Hosseinzadeh, H., Bazzar, B.S.F., Haghi, M.M. 2007. Antibacterial activity of total extracts andessential and essential oil of Nigella Sativa L. Seeds in Mice, Pharmacolgyonline, 2, 429-435.

Kuzovkina, I.N., Szarka, S.Z., Hethelyi, E.,Lemberkovics, E., Szoke, E. 2009. Composition of essential oil in geneti- cally transformed roots of Ruta Graveolens, Rusian Journal of Plant Physiology, vol 56, 846-851.

Levy, S.B., Marshall, B. 2004. Antibacterial resistance worldwide: Causes, challenges and responses, Nature Medicine, 10, 12.

Mancebo, F., Hilje, L., Mora, G.A., Castro, V.H., Salazar, R. 2000. Biological

Nurhaya, M.T., Laina, Z.M.K., Norazian,M.H., May, K.S., Khairul, A.K. 2009. Bioautographic screening for natural quinolone antimicrobial agents from Glycosmic pentaphylla (Retz) DC., Ruta angustifolia (L) Pers. And Lunasia amara Blanco, International Islamic University Malaysia, Kuantan.

Pandey, P., Mehta, A., Hajra, S. 2011. Evaluation of antimicrobial activity of ruta graveolens stem extracts by disc diffusion method, Journal of Phytology, 3(3), 92-95.

Pirouzpanah, S., Rashidi, M.R., Delazar, A., Razavich, S., Hamidi, A. 2006. Inhibitory effect of ruta graveolens l. Extract on Guinea Pig liver aldehyde oxidase, Pharmaceutical Society of Japan, 54(1),9-13.

Pratiwi, S.T. 2011. Mikrobiologi farmasi, 117. Jakarta: Penerbit Erlangga.

Priya, P.S., Sasikumar, J.M., Gowsigan, G.2009. Antibacterial activity of methanol extract of ruta chalapensis (L), quercus infectoria (Oliver) and canthium parviflorum (Lam), Ancient Science of Life, 29(2), 28-31.

Sabir, A. 2005. Aktivitas antibakteri flavonoid propolis trigona sp terhadap bakteri Streptococcus mutans, Majalah Kedokteran Gigi, Vol.36, No.3.

Tenover, F.C. 2006. Mechanism of antimicrobial resistance in bacteria, The American Journal of Medicine, 119 (6A), S3-S10.

Wagner, H. & Bladt, S. 1955. Plant drug analisis, a thin layer chromatography Atlas, Second Edition, 126,129,144,Springer, Munich.

DOI: https://doi.org/10.21831/jps.v19i1.2329


  • There are currently no refbacks.

Copyright (c)

 p-ISSN: 1412-3991 || e-ISSN: 2528-7036

Indexed by:


View My Stats