Determination of total phenolics and fl avonoids content and test  of antioxidant activity of mango leaves honey cultivar

Enny Fachriyah; Mei Riska Wati; Ismiyarto ismiyarto; Purbowatiningrum Ria Sarjono

doi:10.21831/jps.v1i2.77028

Determination of total phenolics and fl avonoids content and test of antioxidant activity of mango leaves honey cultivar

Enny Fachriyah, Universitas Diponegoro, Indonesia
Mei Riska Wati, Universitas Diponegoro, Indonesia
Ismiyarto ismiyarto, Universitas Diponegoro, Indonesia
Purbowatiningrum Ria Sarjono, Universitas Diponegoro, Indonesia

10.21831/jps.v1i2.77028

Abstract

Abstract: This research aims to carry out phytochemical tests which include tests for alkaloids, ﬂavonoids, saponins, phenolics, quinones, saponins, triterpenoids and steroids, total phenolics and ﬂavonoids as well as antioxidant activity of the ethyl acetate and ethanol fractions of honey cultivar mango leaves. The research began with plant determination, sample preparation, phytochemical screening, maceration with 96% ethanol followed by multilevel partitioning using n-hexane and ethyl acetate. Phytochemical screening was carried out on the extracts and fractions obtained. The fractions obtained were determined as total phenolics using the Folin-Ciocalteu method, total ﬂavonoids using the AlCl₃ colorimetric method. Antioxidant activity was determined by the DPPH method. The results of the plant determination conﬁrmed that the sample was Mangifera indica L. honey cultivar, positive for containing ﬂ avonoid, phenolic, saponin, quinone, steroid and triterpenoid compounds. The yields of the ethyl acetate fraction and ethanol fraction were 6.19 and 5.19% respectively. The total phenolic content of the ethyl acetate fraction of honey cultivar mango leaves was respectively 423.7 ± 3.68 mg GAE/g and 48.2 ± 0.139 mg QE/g. The total ﬂavonoid content of the ethanol fraction was 132.35 ± 1.95 mg GAE/g and 27.72 mg QE/g, respectively. The IC₅₀ values of the ethyl acetate fraction and ethanol fraction were 17.83 and 79.73 ppm respectively. Honey cultivar mango leaves can act as an antioxidant agent with a strong category in the ethanol fraction and very strong in the ethyl acetate fraction.Keywords: total phenolics content, total ﬂ avonoids content, antioxidant, Mangifera indica L

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DOI: https://doi.org/10.21831/jps.v1i2.77028