Aktivitas antioksidan dan antibakteri dari alkaloid bakteri endofit Pseudomonas hibiscicola WK asal daun waru (Hibiscus tiliaceus)

Purbowatiningrum Ria Sarjono

doi:10.21831/jps.v30i1.77501

Aktivitas antioksidan dan antibakteri dari alkaloid bakteri endofit Pseudomonas hibiscicola WK asal daun waru (Hibiscus tiliaceus)

Purbowatiningrum Ria Sarjono, Diponegoro University, Indonesia

10.21831/jps.v30i1.77501

Abstract

Telah diperoleh isolat senyawa alkaloid dari bakteri endofit Pseudomonas hibiscicola Wk. Bakteri endofit Pseudomonas hibiscicola Wk adalah bakteri hasil isolasi dari daun waru. Hasil isolasi senyawa alkaloid dikarakterisasi menggunakan kromatografi lapis tipis. Senyawa alkaloid hasil isolasi dilakukan uji kadar total fenol, aktivitas antioksidan. Hasil fitokimia hasil isolasi bakteri endofit Pseudomonas hibiscicola Wk positif mengandung alkaloid, diperkuat dengan nilai Rf sebesar 0,8125 yang mirip dengan nilai Rf alkaloid dari tanaman tapak dara yaitu vindoline (Rf = 0,85). Senyawa alkaloid dari bakteri endofit Pseudomonas hibiscicola Wk memiliki kadar total fenol tertinggi sebesar 2,390 mgek asam galat/gram sampel pada konsentrasi 10.000 mg/L. Hasil tersebut menandakan bahwa pada senyawa alkaloid yang diperoleh terdapat gugus fenol. dNilai IC₅₀ yang diperoleh dari senyawa alkaloid hasil isolasi sebesar 99,70 mg/L yang menunjukkan bahwa alkaloid hasil isolasi mempunyai kemampuan antioksidan yang kuat, serta mampu menghambat pertumbuhan bakteri E.coli dan S.aureus.

Keywords

Daun waru,bakteri endofit, alkaloid, antioksidan, antibakteri

References

Adawiah, A., & Muawanah, A. (2015). Aktivitas Antioksidan dan Kandungan Komponen Bioaktif Sari Buah Namnam. Jurnal Kimia VALENSI, 1(2), 130–136. https://doi.org/http://dx.doi.org/10.15408/jkv.v0i0.3155.

Alferman. (2000). Detection and Identification of Organic Compound. Planum.

Anderson, D. G., Salm, S., Beins, M., & Nester, E. W. (2021). Nester’s microbiology : a human perspective (Tenth). McGraw-Hill. https://doi.org/LK - https://worldcat.org/title/1261497925

Ataei-Azimi, A., Hashemloian, B. D., Ebrahimzadeh, H., & Majd, A. (2008). High in vitro production of ant-canceric indole alkaloids from periwinkle (Catharanthus roseus) tissue culture. African Journal of Biotechnology, 7(16). https://doi.org/10.4314/AJB.V7I16.59172

Badarinath, A. V, Rao, K. M., Chetty, C. M. S., Ramkanth, S., Rajan, T. V. S., & Gnanaprakash, K. (2010). A review on in-vitro antioxidant methods: comparisions, correlations and considerations. International Journal of PharmTech Research, 2(2), 1276–1285. https://doi.org/10.1016/j.jsps.2012.05.002

Bandonienė, D., Murkovic, M., Pfannhauser, W., Venskutonis, P., & Gruzdienė, D. (2002). Detection and activity evaluation of radical scavenging compounds by using DPPH free radical and on-line HPLC-DPPH methods. European Food Research and Technology, 214, 143–147. https://doi.org/10.1007/s00217-001-0430-9

Barrales-Cureño, H. J., Reyes, C. R., García, I. V., & Valdez, L. G. L. (2019). Alkaloids of Pharmacological Importance in Catharanthus roseus. https://doi.org/10.5772/intechopen.82006

Bhore, S. J., Ravichantar, N., & Loh, C. Y. (2010). Screening of endophytic bacteria isolated from leaves of Sambung Nyawa [Gynura procumbens (Lour.) Merr.] for cytokinin-like compounds. Bioinformation, 5(5), 191. https://doi.org/doi: 10.6026/97320630005191

Corona-Bustamante, A., Viveros-Paredes, J. M., Flores-Parra, A., Peraza-Campos, A. L., Martínez-Martínez, F. J., Sumaya-Martínez, M. T., & Ramos-Organillo, Á. (2010). Antioxidant activity of butyl-and phenylstannoxanes derived from 2-, 3-and 4-pyridinecarboxylic acids. Molecules, 15(8), 5445–5459. https://doi.org/10.3390/molecules15085445

Cowan, M. K., & Smith, H. (2021). Microbiology: A Systems Approach, 6th Edition. McGraw Hill. https://doi.org/v9781260451290

Dwidjoseputro. (1998). Dasar-dasar mikrobiologi. Djambatan. https://doi.org/IOS2779.slims-29602

Fessenden, R. J., & Fessenden, J. S. (1986). Kimia organik dasar edisi ketiga. Jilid I. Terjemahan Oleh AH Pudjaatmaka. Jakarta: Erlangga. Hal, 441–442. https://doi.org/10.3390/molecules15085445

Forestryana, D., & Arnida. (2020). Phytochemical screenings and thin layer chromatography analysis of ethanol extract jeruju leaf (Hydrolea spinosa L.). Jurnal Ilmiah Farmako Bahari, 11(2). https://doi.org/http://dx.doi.org/10.52434/jfb.v11i2.859

Gandjar, I. G., & Rohman, A. (2007). Kimia farmasi analisis. Yogyakarta: Pustaka Pelajar, 224, 228.

Gunawan, G., Chikmawati, T., Sobir, S., & Sulistijorini, S. (2016). Fitokimia genus Baccaurea spp. https://doi.org/10.23917/bioeksperimen.v2i2.2488.

Handoyo, D. L. Y. (2020). Pengaruh Lama Waktu Maserasi (Perendaman) Terhadap Kekentalan Ekstrak Daun Sirih (Piper Betle). Jurnal Farmasi Tinctura, 2(1), 34–41. https://doi.org/10.35316/tinctura.v2i1.1546

Hapsari, A. M., Masfria, M., & Dalimunthe, A. (2018). Pengujian kandungan total fenol ekstrak etanol tempuyung (Shoncus arvensis L.). Talenta Conference Series: Tropical Medicine (TM), 1(1), 284–290. https://doi.org/https://doi.org/10.32734/tm.v1i1.75

Illing, I., Safitri, W., & Erfiana, E. (2017). Uji fitokimia ekstrak buah dengen. Dinamika, 8(1), 66–84.

Irianto, K. (2012). Mikrobiologi Menguak Dunia Mikroorganisme, 76-77. Bandung, Yrama Wigya.

Julkunen-Tiitto, R. (1985). Phenolic constituents in the leaves of northern willows: methods for the analysis of certain phenolics. Journal of Agricultural and Food Chemistry, 33(2), 213–217. https://doi.org/https://doi.org/10.1021/jf00062a013

Karlina, C. Y., Ibrahim, M., & Trimulyono, G. (2013). Aktivitas antibakteri ekstrak herba krokot (Portulaca oleracea L.) terhadap Staphylococcus aureus dan Escherichia coli. Lentera Bio, 2(1), 87–93.

Li, D.-L., Li, X.-M., Proksch, P., & Wang, B.-G. (2010). 7-O-Methylvariecolortide A, a new spirocyclic diketopiperazine alkaloid from a marine mangrove derived endophytic fungus, Eurotium rubrum. Natural Product Communications, 5(10). https://doi.org/https://doi.org/10.1177/1934578X1000501014

Maesaroh, K., Kurnia, D., & Al Anshori, J. (2018). Perbandingan metode uji aktivitas antioksidan DPPH, FRAP dan FIC terhadap asam askorbat, asam galat dan kuersetin. Chimica et Natura Acta, 6(2), 93–100. https://doi.org/10.24198/cna.v6.n2.19049

Magagula, N. L., Mohanlall, V., & Odhav, B. (2012). Optimized thin layer chromatographic method for screening pharmaceutically valuable alkaloids of Catharanthus roseus (Madagascar Periwinkle). International Journal of Sciences.

Maryam, S. (2015). Kadar Antioksidan dan IC50 Tempe Kacang Merah (Phaseulus vulgaris L ) yang Difermentasi dengan Lama Fermentasi Berbeda. Prosiding Seminar Nasional MIPA, 347–352.

Maulana, M. (2018). Profil kromatografi lapis tipis (KLT) ekstrak daun bidara Arab (Ziziphus spina-cristi. L) berdasarkan variasi pelarut. Thesis. Universitas Islam Negeri Maulana Malik Ibrahim.

Miroslav, V. (1971). Detection and Identification of Organic Compound. Planum Publishing Corporation and SNTC Publishers of Technical Literatur. https://doi.org/10.1007/978-1-4684-1833-0

Pavlov, A., Kovatcheva, P., Georgiev, V., Koleva, I., & Ilieva, M. (2002). Biosynthesis and radical scavenging activity of betalains during the cultivation of red beet (Beta vulgaris) hairy root cultures. Zeitschrift Für Naturforschung C, 57(7–8), 640–644. https://doi.org/10.1515/znc-2002-7-816

Pelczar, M. J., & Chan, E. C. S. (2006). Dasar-dasar Mikrobiologi. UI Press. Jakarta. https://doi.org/IOS4868.ai:slims-2731

Purba, N., & Putri, N. (2022). Test of Antibacterial Activity from The Combination of Ethanol Extract of Waru (Hibiscus tiliaceus L.) Leaves and Leaf Red Pucuk (Syzygium oleana) Against Salmonella thypi on 2021. JURNAL FARMASIMED (JFM), 4(2), 44–50. https://doi.org/https://doi.org/10.35451/jfm.v4i2.1009

Purwestri, Y. A. (2021). Kemampuan Antibakteri Metabolit Sekunder Bakteri Endofit Tanaman Purwoceng terhadap Escherechia coli. Jurnal Ilmu Hayat, 5(1), 17–24. https://doi.org/http://dx.doi.org/10.17977/um061v5i12021p17-24

Rahmi, R., Herawati, N., & Dini, I. (2016). Isolasi dan identifikasi senyawa metabolit sekunder ekstrak etil asetat kulit batang belimbing wuluh (Averrhoa bilimbi Linn). Jurnal Ilmiah Kimia Dan Pendidikan Kimia, 17(1), 98–107. https://doi.org/doi.org/10.24114/jpkim.v14i2.33706

Robinson, T. (1991). The Organic Constituents of Higher Plants: Their Chemistry and Interrelationships. Cordus Press. https://doi.org/https://doi.org/10.1021/ed040pA983

Saiman, M. Z., Miettinen, K., Mustafa, N. R., Choi, Y. H., Verpoorte, R., & Schulte, A. E. (2018). Metabolic alteration of Catharanthus roseus cell suspension cultures overexpressing geraniol synthase in the plastids or cytosol. Plant Cell, Tissue and Organ Culture, 134(1), 41–5. https://doi.org/10.1007/s11240-018-1398-5

Sarjono, P. R., Anggraeni, A., Monita, A., Asy’ari, M., Ismiyarto, I., Ngadiwiyana, N., & Prasetya, N. (2022). Antibacterial and Antioxidant Activity of Endophytic Bacteria Isolated from Hibiscus tilaceus Leaves. Jurnal Kimia Valensi, 8, 199–210. https://doi.org/10.15408/jkv.v8i2.25686

Sarjono, P. R., Solekhah, P. S., Iskandarsyah, M. R., Asy’ari, M., Ismiyarto, Ngadiwiyana, Basid, N., Prasetya, A., & Andriani, Y. (2023). Antibacteria and Antioxidant Activity of Endophytic Bacteria Isolated from Hibiscus tiliaceus Leaves with zobell as Growth Media. AIP Conference Proceedings, AIP Conf., 1–11. https://doi.org/https://doi.org/10.1063/5.0140270

Sholekhah, P. S. (2021). Isolasi dan Aktivitas Antioksidan Metabolit dari Bakteri Endofit Daun Waru (Hibiscus tiliaceus). Skripsi. Universitas Diponegoro.

Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3), 144–158. https://doi.org/10.5344/ajev.1965.16.3.144

Sirivibulkovit, K., Nouanthavong, S., & Sameenoi, Y. (2018). based DPPH assay for antioxidant activity analysis. Analytical Sciences, 34(7), 795–800. https://doi.org/10.2116/analsci.18P014

Soemiati, A. (2013). Perbandingan Metode Ekstraksi Maserasi dan Sokletasi Terhadap Kadar Piperin Buah Cabe Jawa (Piperis retrofracti fructus). Skripsi.

Sugijanto, N. E. (2011). Produksi Bahan Bioaktif Berkhasiat Obat Menggunakan Jamur Endofit. 5–31.

Surahmaida, S., Rachmawati, A., & Handayani, E. (2020). Kandungan Senyawa Kimia Daun Waru (Hibiscus Tiliaceus) di Kawasan Lingkar Timur Sidoarjo. Journal Pharmasci, 5(2), 39–42. https://doi.org/10.53342/pharmasci.v5i2.167

Suswanti, E. Y. (2021). Isolasi dan Uji Aktivitas Antioksidan Senyawa Alkaloid Bakteri Endofit Pseudomonas azotoformans DP-1. Skripsi. Universitas Diponegoro.

Svendsen, A. B., & Verpoorte, R. (1983). Chapter 19 Colchicine and Related Alkaloids. In Journal of chromatography library (pp. 469–476). https://doi.org/10.1016/S0301-4770(08)60919-7

Tiong, S. H., Looi, C. Y., Hazni, H., Arya, A., Paydar, M., Wong, W. F., Cheah, S.-C., Mustafa, M. R., & Awang, K. (2013). Antidiabetic and antioxidant properties of alkaloids from Catharanthus roseus (L.) G. Don. Molecules, 18(8), 9770–9784. https://doi.org/10.3390/molecules18089770

Wullur, A. C., Schaduw, J., & Wardhani, A. N. K. (2012). Identifikasi alkaloid pada daun sirsak (Annona muricata L.). Jurnal Ilmiah Farmasi (JIF), 3(2), 54–56.

Yoga, I. B. K. W. (2015). Penentuan konsentrasi optimum kurva standar antioksidan; asam galat, asam askorbat dan trolox® terhadap radikal bebas DPPH (2, 2-diphenyl-1-picrylhydrazyl) 0, 1 mM. Prosiding Seminar Nasional MIPA.

DOI: https://doi.org/10.21831/jps.v30i1.77501