KERAGAMAN BAKTERI YANG BERPOTENSI SEBAGAI AGEN PENDEGRADASI LIMBAH MASKER BERBAHAN POLIPROPILEN YANG DIISOLASI DARI PANTAI PARANGTRITIS DAN TANJUNG PASIR

Madda Nur Abidin; Anna Rakhmawati

doi:10.21831/jsd.v12i2.66032

KERAGAMAN BAKTERI YANG BERPOTENSI SEBAGAI AGEN PENDEGRADASI LIMBAH MASKER BERBAHAN POLIPROPILEN YANG DIISOLASI DARI PANTAI PARANGTRITIS DAN TANJUNG PASIR

Madda Nur Abidin, Department of Biology Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta, Yogyakarta, Indonesia, Indonesia
Anna Rakhmawati, Department of Biology Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta, Yogyakarta, Indonesia, Indonesia

10.21831/jsd.v12i2.66032

Abstract

Penelitian ini bertujuan untuk mengetahui karakteristik, jenis bakteri yang dapat mendegradasi limbah masker nonmedis duckbill hitam berbahan polipropilen, serta aktivitas degradasi polipropilen oleh 10 isolat bakteri pada limbah masker yang ditemukan di pesisir Pantai Parangtritis, DI Yogyakarta dan Tanjung Pasir, Banten. Penelitian dilakukan dengan pengukuran nilai optical density isolat bakteri, uji degradasi zona bening, karakterisasi secara morfologi, fisiologi, serta profile matching dengan karakter bakteri acuan sesuai Bergeys’s Manual of Determinative Bacteriology dan referensi ilmiah pendukung untuk menentukan dugaan genus isolat. Hasil karakterisasi fenotipik dikonstrusikan dengan dendogram untuk mengetahui indeks similaritas. Hasil penelitian diketahui bahwa kemampuan isolat bakteri limbah masker mendegradasi polipropilen dalam kategori sedang (diamater 1,0 – 2,5 mm). Karakter isolat yaitu bakteri gram positif dan negatif; berbentuk coccus dan basil; bakteri dominan tidak membentuk endospora, bentuk semua koloni circular; elevasi flat dan raised; margin undulate, entire, dan lobate; warna putih susu dan putih kekuningan; dan hasil uji biokimia beragam. Hasil dendogram didapatkan 3 jenis (genus) bakteri yaitu Pseudomonas sp., Rhodococcus sp. dan Halomonas sp.

Keywords

Bakteri Pendegradasi Polipropilen, Biodegradasi, Identifikasi , Limbah Masker

Full Text:

PDF

References

Al-Jailawi, M. H., Ameen, R. S., & Al-Saraf, A. A. (2015). International Journal of Advanced Research in Biological Sciences Polyethylene degradation by Pseudomonas putida S3A. Journal of International Environmental Application and Science. 2(1), 90–97. Retrieved from www.ijarbs.com.

Auta, H.S., C.U. Emenikea, B. Jayanthia , S.H. Fauziah. (2018). Growth Kinetics and Biodeterioration of Polypropylene Microplastics by Bacillus sp. and Rhodococcus sp. Isolated from Mangrove Sediment. Marine Pollution Bulletin, 127, 15-21.

Cappuccino, J. G. & Sherman, N. (2014). Manual Laboratorium Mikrobiologi. 8th edn. Jakarta : Penerbit Buku Kedokteran EGC.

Devi, Deepa, Kartikey Kumar Gupta, and Deepanshu Rana. (2016). Isilation and Screening of Low Density Polyethylene (LDPE) Degrading Bacterial Strains from Waste Disposial Sites. World Journal of Pharmaceutical Research, 5(11), 1633-1643.

Ding L, Mao R, Ma S, Guo X, Zhu L (2020). High Temperature Depended on the Ageing Mechanism of Microplastics Under Different Environmental Conditions and Its Effect on the Distribution of Organic Pollutants. Water Research, 174: 115634.

Gupta, Kartikey Kumar, Devi, Deepa, and Deepanshu Rana. (2016). Isolation and Sreening of Low Density Polyethylene (LDPE) Degrading Bacterial Srains from Waste Dispsosal Sites. World Journal of Pharmaceutical Research, 5(11), 1633-1643. Retrieved from https://10.20959/wjpr201611-7377.

Guzik, U., Hupert-Kocurek, K. & Wojcieszyńska, D. (2013). Intradiol dioxygenases – The Key Enzymes in Xenobiotics Degradation, In: Chamy, R. (Ed.), Biodegradation of Hazardous and Special Products, Intech Open, 129–153. Retrieved from http://dx.doi. org/10.5772/56205.

Hadioetomo R.S. (1993). Mikrobiologi Dasar Dalam Praktek. Jakarta : Gramedia.

Hammami, Khouloud , Yasmine Souissi, Ameur Cherif, Mohamed Neifar. (2023). Sustainable bioconversion of synthetic plastic wastes to polyhydroxyalkanoate (PHA) bioplastics: recent advances and challenges. MedCrave Online Journal of Applied Bionics and Biomechanics, 7(1), 48-62.

Handayani, K., Ekowati, C. N., & Pakpahan, M. (2013). Karakterisasi fisiologi dan pertumbuhan isolat bakteri Bacillus thuringiensis dari tanah naungan di lingkungan Universitas Lampung. Disampaikan pada Seminar Nasional Sains dan Teknologi V, Lembaga Penelitian Universitas Lampung.

Iturbe, R, and Lopez, J. (2015). Bioremediation for A Soil Contaminated with Hydrocarbons. Journal of Petroleum & Environmental Biotechnology, 6(2), 1.

Jangra, Mukesh R., K. S Nehra, Garima, Kajal, Monika, Sonia, Preet, and Sumit Jangra. (2020). Assessment of Plastic Degrading Ability of Microbes Isolated from Local Plastic Dumping Sites. Biosc Biotech Res Comm, 13(4), 2360-2364.

Jeon, J. M., Park, S. J., Choi, T. R., Park, J. H., Yang, Y. H., & Yoon, J. J. (2021). Biodegradation of polyethylene and polypropylene by Lysinibacillus species JJY0216 isolated from soil grove. Polymer Degradation and Stability, 191. Retreived from https://doi.org/10.1016/j.polymdegradstab.2021.109662.

Juliandi, Muhammad Diki, Anthoni Agustien, Azzura Ligo, dan Akmal Djamaan. (2020). Characterization of Polypropylene Synthetic Plastic-Degrading Bacteria from Seawater Samples and Their Decomposition Profiles. IOSR Journal Of Pharmacy And Biological Sciences (IOSR-JPBS). 15(5), 50-58.

Kaushal, J., Khatri, M., Arya, K.S. (2021). Recent insight into enzymatic degradation of plastics prevalent in the environment: A mini—Review. Clean Eng. Technology, 2, 100083.

Kurnia K., Sadi N.H, Jumianto S. (2015). Isolation and Characterization of Pb Resistant Bacteria from Cilalay Lake, Indonesia. Aceh Int. J Sci. Technology, 4(3), 83-87.

Locke, T., S. Keat, A. Walker and R. Mackinnon. (2013). Microbiology and Infectius Diseases on The Move.

Mira, P., Yeh, P., & Hall, B. G. (2022). Estimating Microbial Population Data from Optical Density. PLOS ONE, 17(10).

Miri, S., Saini, R., Davoodi, S., Pulicharla, R., Brar, S., & Magdouli, S. (2022). Biodegradation of Microplastics: Better Late Than Never. Chemosphere, 286, 131670.

Nathania, H. B. (2013). Studi potensi isolat kapang Wonorejo Surabaya dalam mendegradasi polimer bioplastik poly, 2(2), 1-11.

Nisida H, Tokiwa Y. (1993). Distribution of Poly (β-hydroxybutyrate) and Poly (ε-caprolacton) Aerobic Degrading Microorganisms in Different Environments. J. Environ. Polym. Degrad, 1: 227-233.

Oliveira, J., Belchior, A., da Silva, V., Rotter, A., Petrovski, Ž., Almeida, P., Lourenco, N., & Gaudencio, S. (2020). Marine environmental plastic pollution: mitigation by microorganism degradation and recycling valorization. Frontiers in Marine Science, 7, 567126.

Özdemir, S.; Akarsu, C.; Acer, Ö.; Fouillaud, M.; Dufossé, L.; Dizge, N. (2022). Isolation of Thermophilic Bacteria and Investigation of Their Microplastic Degradation Ability Using Polyethylene Polymers. Microorganisms, 10, 2441. Retrieved from : https://doi.org/10.3390/10122441.

Pikoli, Megga Ratnasari, Festy Auliyaur Rahmah, Arina Findo Sari, Puji Astuti, dan Nur Amaliah Solihat. (2020). Memancing Mikroba dari Sampah : Isolasi Mikroorganisme Pendegradasi Mikroplastik dari Tempat Pembuangan Akhir (TPA) Sampah. Jakarta : Kinzamedia.

Pollitt, Eric J. G. Stephen P. Diggle. (2017). Defning motility in the Staphylococci. Cellular and Molecular Life Sciences, 74:2943–2958.

Putri, A.M. dan Kurnia, P. (2018). Identifikasi Keberadaan Bakteri Coliform dan Total Mikroba dalam Es Dung-dung di Sekitar Kampus Universitas Muhammadiyah Surakarta. Media Gizi Indonesia, 13( 1), 41–48

Qodriyah, Nur Romadhona Lailatul. (2022). Biodiversitas Bakteri Pendegradasi Polipropilena dari Komunal Tempat Pemrosesan Akhir (TPA) Sampah Kota Probolinggo dan Potensinya untuk Biodegradasi Limbah Masker Medis . Thesis. Malang: Universitas Negeri Malang.

Rafiq, S., Fahmida, F & Shahina, S.K. (2018). Biodegradation of Low Density Polyethylene (LDPE) by Halophilic Bacteria Isolated from Solar Saltpans Kovalam, Chennai. An International Quarterly Scientific Journal, 17(4).

Riandi, M. I., Kawuri, R., & Sudirga, S. K. (2017). Potensi Bakteri Pseudomonas sp. dan Ochrobactrum sp. yang Diisolasi dari Berbagai Sampel Tanah dalam Mendegradasi Limbah Polimer Plastik Berbahan Dasar High Density Polyethylene (HDPE) dan Low Density Polyethylene (LDPE). Simbiosis Journal of Biological Sciences, 5(2), 58. DOI : 10.24843/JSIMBIOSIS.2017.v05.i02.p05.

Sangkham S. (2020). Face mask and medical disposal during the novel COVID-19 pandemic in asia. Case Stud Chem Environ, 2, 100052. dalam Lubriyana, Triyani, Nurjazuli, dan Dewanti, Nikie Astorina Yunita. (2022). Gambaran Pengelolaan Limbah Masker Sekali Pakai oleh Rumah Tangga pada Masyarakat di Kota Semarang. Jurnal Riset Kesehatan Masyarakat, 2(2).

Skariyachan, S., Patil, A. A., Shankar, A., Manjunath, M., Bachappanavar, N., & Kiran, S. (2018). Enhanced Polymer Degradation of Polyethylene and Polypropylene by Novel Thermophilic Consortia of Brevibacillus sp. and Aneurinibacillus sp. Screened from Waste Management Landfills and Sewage Treatment Plants. Polymer Degradation and Stability, 149, 52–68. Retrieved from https://doi.org/10.1016/j.polymdegradstab.2018.01.018.

Sriningsih, Atik dan Maya Shovitri. (2015). Potensi Isolat Bakteri Pseudomonas sebagai Pendegradasi Plastik. Jurnal Sains dan Seni ITS, 4 (2), 2337-3520.

Sugiarto, A. T., & Suherman. (2021). Menangani Limbah Medis Covid-19 Dengan Teknologi Plasma Nanobubble. Lipi.Co.Id. Retrieved from http://bpi.lipi.go.id/menangani-limbah-medis-covid-19-dengan-teknologi-plasma-nanobubble.

Trikurniadewi, N., Khiftiyah, A. M., Sari, S. K., Ifananda, W., & Suryani, D. I. (2022). Isolates Of Polypropylene-Degrading Bacteria. Jurnal Riset Kesehatan, 11(2), 89–95.

Vianti, Rizky Okta, Melki, Rozirwan, dan Purwiyanto, Anna Ida Sunaryo. (2020). Purifikasi dan Uji Degradasi Bakteri Mikroplastik dari Perairan Muara Sungai Musi, Sumatera Selatan. Maspari Journal, 12(2), 29-36.

Wei, R., & Zimmermann, W. (2017). Microbial enzymes for the recycling of recalcitrant petroleum‐based plastics: how far are we? Microbial Biotechnology, 10(6), 1308-1322.

Xu, Elvis Genbo dan Ren, Zhiyong Jason. (2021). Preventing Masks from Becoming the Next Plastic Problem. Front Environ Science Engineering, 15(6), 125.

Zampolli, Jessica , Alessandro Orro, Daniele Vezzini, & Patrizia Di Gennaro. (2022). Genome-Based Exploration of Rhodococcus Species for Plastic-Degrading Genetic Determinants Using Bioinformatic Analysis. Microorganisms , 10, 1846.

Zeenat, Elahi, A., Bukhari, D., Shamim, S., & Rehman, A. (2021). Plastics Degradation by Microbes: a Sustainable Approach. Journal of King Saud University - Science, 33(6), 101538.

DOI: https://doi.org/10.21831/jsd.v12i2.66032