Inducing An Axillary Bud of Dendrobium Red Emperor ‘Prince’ With An Addition of Bap in Vitro

Authors

  • Mistri Septasari Universitas Negeri Yogyakarta
  • Ixora Sartika Mercuriani Universitas Negeri Yogyakarta

DOI:

https://doi.org/10.21831/ijobi.v1i2.216

Keywords:

Dendrobium Red Emperor ‘Prince’ orchid, BAP, the time of appearance of buds, the growth of the header, root growth

Abstract

The purpose of this study is to determine the effect of the addition of BAP and the position of nodus on the formation of axillary shoots and to know the optimum concentration of BAP for the induction of Dendrobium Red Emperor  'Prince' axial shoots. The research design used is a Complete Factorial Randomized Design consisting of one treatment, namely: concentration of growing regulatory substances (ZPT) BAP (0ppm, 1 ppm, 2 ppm, and 3 pmm), each treatment repeated 5 times. The explant of the nodus were taken from plants produced by previous in vitro culture having 5 nodi. The basic medium used is New Phalaenopsis (NP) + Coconut Water + 1 ppm 2.4-D). The growth of axillary bud growth is measured based on the time of the bud emergence,  growth of crown, and that of  root. Data obtained were then analyzed using ANOVA. If there was a significant difference, the analysis was continued with a test of DMRT with a significant level of 5%. The results showed the addition of concentrations of 1 ppm and 2 ppm BAP influenced  the time of the bud emergence,  growth of crown, and that of  root. The optimum concentration of BAP to induce the orchid axillary shoots is 2 ppm.

Downloads

Download data is not yet available.

References

Akbar, M. A., Eny F., Sapto I., & Toni H. (2017). Induksi Tunas, Multiplikasi dan Perakaran Gyrinops versteegii (Gilg.) Domke Secara In Vitro. Jurnal Pemulihan Tanaman Hutan. Vol. 11 No. 1.

Astutik & Andayani. (2005). Pengaruh Pemberian BAP dan NAA terhadap Pertumbuhan Krisan (Chrysanthemum morifolium, Ram). Jurnal Kultur Jaringan Biota X (3): 31-35.

Ayuningsari, I., S. Rosniawati, Y. Maxiselly, & I. R. D. Anjarsari. (2017). Pengaruh Konsentrasi Benzyl Amino Purine Terhadap Pertumbuhan Beberapa Klon Tanaman Teh (Camelia sinensis L.) O. Kuntze) Belum Menghasilkan di Dataran Rendah. Jurnal Kultivasi. Vol. 16 (2) :356-361.

Bakar, Meklin. (2015). Penggunaan BAP dan Kinetin Pada Induksi Tunas Dari Protocorm Anggrek Dendrobium (Dendrobium sp.) Pada Kultur In Vitro. Manado: Universitas Sam Ratulangi.

Bakar, Meklin. (2015). Penggunaan BAP dan Kinetin Pada Induksi Tunas Dari Protocorm Anggrek Dendrobium (Dendrobium sp.) Pada Kultur In Vitro. Manado: Universitas Sam Ratulangi.

Beck, C. B. (2010). An Introduction to Plants Stucture and Development. Cambridge: Cambridge Univercity Press.

Berlinasari, T. & Muhammad Muryono. (2019). Identifikasi Morfologi Akar Terhadap Toleransi Salin Pada Fase Vegetatif di Beberapa Kultivar Tanaman Padi (Oryza sativa L.). Surabaya : Institut Teknologi Sepuluh Nopember.

Brault, Mathias & Maldiney Regis. (1999). Mechanism of Cytokinin Action. Laboratoire de physiologie du développement des plantes, université Pierre-et-Marie-Curie (CNRS, UMR 7632). Plant Physiol. Biochem. 37 (5): 403−412.

Bredmose, N. & J. Hunsen. (1996). Topophysis Affects The Potential of Axillary Bud Growth, Fress Biomass Accumulation and Spesifics Fresh Weight in Single Stem Roses (Rosa hybrida L.). Annals of Botany. 78:215-222.

BPS. (2013). Produksi Tanaman Hias di Indonesia 1997-2011. http://www.bps. go.id/tabsub/view.php?kat=3&tabel=1&daftar=1&id_subyek=55&notab=19. Diakses pada tanggal 08 Juli 2021.

Dwiyani, Rindang. (2016). Induksi Organogenesis pada Mikropropagasi Anggrek Vanda tricolor Lindl. Serta Deteksi Variasi Genetik Hasil Perbanyakan dengan RAPD. Denpasar: Universitas Udayana.

Fathurrahman, T. R., Ahmad S. Nst, & Gunawan S. (2012). Multiplikasi Tunas Pucuk Tomat (Lycopersicum esculentum Mill) dengan Menggunakan Benzyl Amino Purine (BAP) dan Napthalene Acetic Acid (NAA) Secara In Vitro. Jurnal Agroteknologi. Vol.1 (1): 1-12.

George, E.F., M. A. Hall & G. D. Klerk. (2008). Plant Propagation by Tissue Culture (3rd ed). Wageningen : Springer.

Hardjo, P. H. (1994). Organogenesis Langsung dan Kalogenesis pada Kultur Kedelai (Glysine max L. Merril) dan Glysine tomentella H. dalam Medium MS dan PCL-2 Termodifikasi. Tesis. Program Pasca Sarjana. Bogor: IPB.

Heyne, K. (1987). Tumbuhan Berguna Indonesia, Jilid 1-4, 1247. Jakarta: Yayasan Sarana Wana Jaya.

Huyen, D.D. (2003). Nervilia Comm. Ex Gaundich. In: Plant Resources of South-East Asia. No 12(3). Medicinal and Pousionous Plants 3. Bogor: Prosea Foundations.

Kieber, Joseph J. (2002). The Arabidopsis Book: Cytokinins. American Society of Plant Biologist. Carolina: Biology Department, University of North Carolina.

Mahadi, Imam. 2016. Multifikasi Tunas Anggrek Larat (Dendrobium phalaenopsis Fitzg) Dengan Pemberian Hormon BAP dan 2,4-D Terhadap Pertumbuhan Secara In Vitro. Riau : Universitas Riau.

Mansur, Irdika & Muhd Indarwan K. (2019). Teknik Pembibitan Kayu Putih (Melaluca Cajuputi) Secara Vegetatif di Persemaian Perusahaan Batubara PT Bukit Asam (Persero) Tbk. Jurnal Silvikultur Tropika. Vol.10 No.01: 21-28.

Mastuti, L., Rosita P. S., & Sepdian L. A. (2018). Multiplikasi Tunas Tanaman Kapas (Gossypium sp.) Varietas Kanesia 15 Menggunakan Kombinasi BAP dan NAA Secara In Vitro. Journal Of Applied Agricultural Sciences. Vol.2 No.2 :171-181.

Nofiyanto, R. T., F. Kusmiyati, & Karno. (2019). Peningkatan Kualitas Planlet Tanaman Pisang Raja Bulu (Musa paradisiaca) dengan Penambahan BAP dan IAA pada Media Pengakaran Kultur In Vitro. Jurnal Agro Complex. 3 (3) : 132-141.

Onuoch, C. I. & N. I. C. Onwubiku. (2007). Micropropagation of Cassava (Manihot Esculenta Crantz) Using Different Concentration of Benzylaminophurin (BAP). Journal of Engineering an Applied Science. 2(7) :1229-1231.

Pamungkas, S. S. T. (2015). Pengaruh Konsentrasi NAA dan BAP Terhadap Pertumbuhan Tunas Eksplan Tanaman Pisang Cavendish (Musa paradisiaca L.) Melalui Kultur In Vitro. Gontor AGROTECH Science Journal. Vol.2 No.1.

Prihatmanti, D. & Mattjik N. A. (2004). Zat Pengatur Tumbuh NAA dan BAP serta air Kelapa untukMenginduksi Organogenesis Tanaman Athurium. Buletin Agronomi. 32 (1):20-25.

Purnamasari, A., Aloysius S., Lili S. & Ixora S. M. (2020). Optimasi Media Kultur In Vitro Anggrek Dendrobium nobile Berbasis Pupuk Dengan Penambahan Air Kelapa dan Vitamin B1. Jurnal Penelitian Saintek. 25 (2): 157-172.

Rianawati, S., A. Purwito, B. Marwoto, R. Kurniati, & Suryanah. (2009). Embriogenesis Somatik Dari Eksplan Daun Anggrek Phalaenopsis sp. L. Jurnal Agronomi Indonesia. 37 (3): 240-248.

Rohayati, Euis. (2009). Teknik Perbanyakan Cepat Anthurium dengan Induksi Tunas Aksiler secara In Vitro. Buletin Teknik Pertanian. 14 (1): 1-5.

Sandra, Edhi. (2004). Kultur Jaringan Anggrek Skala Rumah Tangga. Jakarta: Agromedia Pustaka.

Santoso, U. & F. Nursandi. (2004). Kultur Jaringan Tanaman. Malang: UMM Press.

Downloads

Published

2023-10-31