The Influence of Al3+ Metal Ion in AlCl3 Compound On α- Amylase Enzyme Activity

Authors

  • Choirul Chasanah Universitas Negeri Yogyakarta, Indonesia
  • Das Salirawati Universitas Negeri Yogyakarta, Indonesia

DOI:

https://doi.org/10.21831/ijce.v3i2.43512

Abstract

The influence of  Al3+ metal ion in  AlCl3 towards α-amylase enzyme activity have been studied. The activity of the α-amylase enzyme on potato starch substrate was determined using the DNS method (3,5-dinitrosalicylic acid) at optimum conditions. The data analysis was conducted in a descriptive qualitative manner. The results obtained that α-amylase enzyme activity with potato starch substrate was optimum at  20 mg/mL with pH 7.2, incubation temperature 37ËšC, incubation time 15 minutes, and enzyme concentration of 40 mg/mL with an average enzyme activity value of 0.0079 mg/mL/minute at 37ËšC. However, after the addition of Al3+ metal ion, the average value of enzyme activity decreased. These means that empirically Al3+ metal ion has a tendency to be inhibitory to the α-amylase enzyme activity on potato starch substrates.

Downloads

Published

2021-08-30

How to Cite

[1]
Chasanah, C. and Salirawati, D. 2021. The Influence of Al3+ Metal Ion in AlCl3 Compound On α- Amylase Enzyme Activity. Indonesian Journal of Chemistry and Environment. 3, 2 (Aug. 2021), 45–52. DOI:https://doi.org/10.21831/ijce.v3i2.43512.

Issue

Vol. 3 No. 2 (2020): DECEMBER 2020

Section

Articles

Citation Check

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright under a Creative Commons Attribution–ShareAlike License (CC BY SA) that allows others to share: copy, and redistribute the material in any medium or format, Adapt: remix, transform, and build upon the material, for any purpose, even commercially.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.