PENGARUH USIA DAUN JELATANG (Urtica dioica L) TERHADAP KADAR VITAMIN C MENGGUNAKAN SPEKTROFOTOMETRI ULTRAVIOLET

Zuhairiah Nasution, Universitas Sari Mutiara Indonesia, Indonesia
Siti Maimunah, Universitas Sari Mutiara Indonesia, Indonesia
Amila Amila, Universitas Sari Mutiara Indonesia, Indonesia

Abstract


Daun jelatang (Urtica dioica L) merupakan spesies yang paling banyak dikenal dalam genus urtica dan memiliki banyak manfaat bagi masyarakat. Daun jelatang dapat dimanfaatkan secara luas baik untuk pengobatan, kosmetika maupun untuk dikonsumsi. Penelitian ini bertujuan untuk mengetahui pengaruh usia daun jelatang terhadap kadar vitamin C-nya dengan metode spektrofotometri ultraviolet. Sampel yang digunakan adalah daun jelatang segar yang tua dan muda. Penelitian diawali dengan analisis kualitatif vitamin C pada sampel daun jelatang tua dan muda kemudian dilanjutkan dengan analisis kuantitatif menggunakan alat spektrofotometer pada panjang gelombang 265 nm.  Dari penelitian yang dilakukan diperoleh bahwa kadar vitamin C pada daun jelatang tua lebih tinggi daripada kadar vitamin C daun jelatang muda. Hasil uji beda dengan paired sample t-test menunjukkan bahwa terdapat perbedaan yang bermakna yang ditunjukkan dengan nilai Sig.(2-tailed) 0,00 (0,00<0,05) sehingga dapat disimpulkan bahwa usia daun berpengaruh signifikan terhadap kadar vitamin C Daun Jelatang.

THE EFFECT OF STINGING NETTLE (Urtica dioica L) LEAVES’ AGE ON VITAMIN C LEVELS USING ULTRAVIOLET SPECTROPHOTOMETRY


Nettle leaf (Urtica dioica L) is the most widely known species in the genus Urtica and has many benefits for the community. Nettle leaves can be used widely for medicine, cosmetics, and consumption. This study aims to determine the effect of nettle leaf age on vitamin C levels using ultraviolet spectrophotometry. The samples used were old and young fresh nettle leaves. The study began with a qualitative analysis of vitamin C in old and young nettle leaves samples, followed by quantitative analysis using a spectrophotometer at a wavelength of 265 nm. The research conducted found that vitamin C levels in old nettle leaves were higher than vitamin C levels in young nettle leaves. The results of the different tests with paired sample t-test showed a significant difference as indicated by the Sig. (2-tailed) value of 0.00 (0.00 <0.05) so that it could be concluded that leafage had a significant effect on vitamin C levels in nettle Leaf.


Keywords


daun jelatang, vitamin C, usia

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DOI: https://doi.org/10.21831/jps.v26i2.43615

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