SITOTOXIC TESTS OF SOME MONO COMPONENTS OF COCON HYDROXES ON CANCER CELL LINE T47D

Retno Arianingrum, FMIPA UNY, Indonesia
Indyah Sulistyo Arty, FMIPA UNY, Indonesia
Sri Atun, FMIPA UNY, Indonesia

 10.21831/jps.v16i2.3387

Abstract


The aim of this research was to study cytotocix activity from some mono para hydroxy compounds (MPHC) i.e. MPHC A, MHPC B, MPHC MPHC D, and MPHC E against T47D cancer cell lines. Those compounds were synthesized from benzaldehyde derivatives and acetofenon derivates through cross aldol reaction under acid condition. Separation and purification of these compounds were conducted by recrystallization technique using suitable eluent. Identification and structure elucidation was done by comparing the data of thin-layer chromatography (TLC) with marker (compounds that have been found previously), and also used the data of IR spectrum. Each compound then was performed cytotoxicity test by in vitro against T47D cells using MTT assay. Observation of cell morphological changes was observed using phase contrast microscopy. The results showed that the compound MPHC A, MPHC B, MPHC C, and MPHC D had cytotoxic activity against T47D cancer cell line, while the compound MPHK E did not have cytotoxic activity. The highest toxicity was MPHK A with LC50= 66.44 Pg/mL. The presence of hydroxyl groups contributed to the enhancement of cytotoxic effect.


Keywords


MPHC compounds ; cytotoxic; and T47D cell.

Full Text:

PDF

References


Afzal S., Asad M.K, Rumana Q.F, Ansari, Muhammad F.N, and Syed S.S. (2008). redox behavior of anticancer chalcone on a glassy carbon electrode and evaluation of its interaction parameters with DNA. Int. J. Mol. Sci., 9, 1424-1434.

Bohm, T., (1998). An old paradigm for treating cancer and other disease in 21 st century, Cane and Met rev, 12: 149-154.

Boumendjel A., Ronot X., Boutonnat. (2009). Chalcone derivatives acting as cell cycle blockers: potensial anticancer drugs? J Curr Drug Targets. Apr;10 (4):363-71.

Goldie, J.H. (2001). Drug resistance in cancer: A perspective. Cancer and Metastasis Rev, 20: 63-68.

Indyah S.A., Henk T., Samhudi, Sastrohamidjojo, and Henk an der Goot. (2000). Synthesis of benzylideneacetophenones and their inhibition of lipidperoxidation. Eur. J. Med. Chem., 35, 449-457.

Indyah S.A. (2007). Cyclooxygenase inhibitory activity of benzilideneaceto-fenone analogue. Recent development in curcumin pharmacochemistry. Procedding of International Symposium on Recent Progress in Curcumin Research, 11-12 September.

King, R.J.B. (2000). Cancer biology, 2nd ed. England: Pearson Education Limited.

Lee, Y.S., Lim, S.S., Shin, K.H., Kim, Y.S., Ohuchi, K., Jung, S.H. (2006). Antiangiogenic and antitumoractivities of 2- hydroxy-4- methoxychalcone. Biol. Pharm. Bull. 29, 1028-1031.

Mathivadani, P., Shanthi, P., and Sachdanandam, P. (2007). Apoptotic effect of semecarpus anacardium nut extract on T47D cancer cell line. Cell. Biol. Int., 31, 1198-1206.

Sasayama, T.; Tanaka, K.; Mizukawa, K.; Kawamura, A.; Kondoh, T.; Hosoda, K.; Kohmura, E. (2007). Trans-4-lodo,4- boranyl-chalcone induces antitumor activity against malignant glioma cell lines in vitro and in vivo. J. Neu-Onc., 85, 123-132.

Ye, C.L., Liu, J.W., Wei, D.Z., Lu, Y.H., Qian, F. (2004). In vitro antitumor activity of 2, 4-dihydroxy-6-methoxy-3, 5- dimethylchalcone against six established human cancer cell lines. Pharmacol. Res., 50, 505-510.

________. (2005). In vivo antitumor activity by 2, 4-dihydroxy-6-methoxy-3, 5- dimethylchalcone in a solid human carcinoma xenograft model. Canc. Chemo.Pharm., 55, 447-452




DOI: https://doi.org/10.21831/jps.v16i2.3387

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c)



 p-ISSN: 1412-3991 || e-ISSN: 2528-7036

Indexed by:

     

View My Stats